Part:BBa_K4817007:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4817007

Figure 2. pET-28a-DspB-C-His plasmid

Figure.3a The detachment effect of DspB on DH5α biofilm(24h);
Figure.3b The detachment effect of DspB on BL21(DE3)-pET28a biofilm(24h)
Figure.3c The detachment effect of DspB on SRB biofilm（7h）

DspB has good degradation effects on the biofilms of E.coli BL21(DE3), E.coli DH5α and SRB.

Figure.4 Effect of BL21(DE3)-DspB on biofilm formation

The engineered bacterial biofilms were collected and plated. The number of attached bacteria to the engineered bacterial biofilm containing the plasmid pET-28a-DspB was minimal, indicating that DspB can inhibit biofilm formation.

Figure.5 α-GC Activity Assay results of DspB(left:p-nitrophenol concentration(nmol/mL) assay; right: GC activity)

The enzyme activity of the crude protein extract was measured, and the results showed that the enzyme activity of DspB was low.

User Reviews

UNIQ1681c374464b20c9-partinfo-00000001-QINU UNIQ1681c374464b20c9-partinfo-00000002-QINU